I am trying to process NanoBRET assay data to analyze competition between Ternary Complex (TC) formation and binary binding between Chimeric Targeted Molecule and weaker affinity interacting species using R. I could not locate the correct library function that helps perform the biphasic dose-response curve fit using the following formula. Can someone direct me to the appropriate R Library if available?
Concn CompoundX CompoundX
0.00001 0.309967 0.28848
0.000004 0.239756 0.386004
0.0000015 0.924346 0.924336
0.00000075 1.409483 1.310479
0.00000025 2.128796 2.007222
0.0000001 2.407227 2.371517
3.75E-08 2.300768 2.203162
1.63E-08 1.826203 1.654133
6.25E-09 0.978104 1.06907
2.5E-09 0.483403 0.473238
1.06E-09 0.235191 0.251971
4.06E-10 0.115721 0.114867
1.56E-10 0.06902 0.053681
6.25E-11 0.031384 0.054416
2.66E-11 0.023007 0.028945
1.09E-11 0.003956 0.020866
Plot generated in GraphPad PRISM using biphasic dose-response equation.
I needed to answer my questions by following further links in the article suggested by @I_O. Apparently the bell-shaped response curve which I thought looked more like the "bell-shaped" model described in the skimpy Prism documentation is precisely what the referenced article was calling "biphasic". See: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4660423/pdf/srep17200.pdf
The R code to do the fitting is in the supplemental material referenced at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4660423/#S1
That Supplement goes on to compare a variety of model variations and constraints, so it should be read, digested and followed more closely than space permits here.