Problem after Create Setup file Python Project (PyQt5) with cx Freez in user

Question

Several of my own projects, which are separate independent programs, were created by myself after converting the Python file to an exe file in my own system and virtual machines. I encounter an unknown error that I have not encountered before. The program specifications were created in Python with PyQt5 and converted to an executable .exe file by cxFreez. The content of the file is like the example below:

- /Application_Folder
---- /assestFolder
---- /libFolder 
---- mainFile.exe
---- file01.exe [or file01.py]
---- file02.exe [or file02.py]
---- file03.exe [or file03.py]
---- media_rc.py # convert and generated from pyrcc5 in pyqt5 tools
---- media.qrc

I also output by default in my system that this problem persisted and I created a new virtual machine (env) and installed all the required packages in it first and then output by a setup.py file whose contents are as follows :

import sys
from cx_Freeze import setup, Executable

build_exe_options = {
    "packages": ["os", "PyQt5", "Blockthon", "requests", "random"],
    "excludes": ["tkinter"],
    "bin_includes": ["sip"],
    "bin_path_includes": ["./env/Lib/site-packages/PyQt5/Qt/bin/"],
    "bin_path_excludes": ["./env/"],
    "include_files": ['assestFolder/', 'libFolder/']
}

base = None
if sys.platform == "win32":
    base = "Win32GUI"

exe = Executable(
    script="mainFile.py",
    base=base,
    target_name = "mainFile.exe",
    target_dir = "output"
)

setup(
    name="name_of_your_application",
    version="0.1",
    description="Your application description",
    options={"build_exe": build_exe_options},
    executables=[exe]
)

The image is wrong