My message box launches automatically without me clicking the button first when I run it in Pycharm.
from tkinter import *
from PIL import ImageTk,Image
from tkinter import messagebox
root = Tk()
root.title('Frame')
root.iconbitmap('D:\Tkinter\Frame.ico')
def popup():
messagebox.showinfo("Popup","You have clicked a button!")
Button(root, text = 'Click Me!',commmand=popup()).pack()
root.mainloop()
In Button declaration, you are calling your function instead of passing a callback to it.
There is also a typo in a word 'command' - you wrote it with 3x m.
So, you should declare your button as:
How to create a basic button