Problems after creating access rule in ASP.NET Configuration security

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I get this message after creating an access rule in ASP.NET Configuration security tab:

"A network-related or instance-specific error occurred while establishing a connection to SQL Server. The server was not found or was not accessible. Verify that the instance name is correct and that SQL Server is configured to allow remote connections. (provider: SQL Network Interfaces, error: 26 - Error Locating Server/Instance Specified)

When I run the application locally everything work as it should, but when I upload it to my web server, hell breaks loose... The access rule I created is for a sub folder and is taking care that only members are allowed to access it. And as I said, everything work locally. I am using the membership model. When the rule is not active, everything work, except that then the member area is open of course...

Any thoughts?

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