Running BootSector on VirtualBox

Question

I made a simple Operating System and I have problem with running kernel, Actually Kernel Prints Nothing.

BootSector :

bits 16
org 0x7c00 ; 0x7c00 > Boot & 0x7c00 + 512 (0x7e00) > Kernel

%ifdef WITH_BPB
%include "BIOSParameterBlock.inc"
%endif

boot_continue:

    mov ax, 0x1000      
    mov ss, ax      ;SS:SP > 0x1000:0xfffe (0x1fffe)
    mov ax, 0xfffe
    mov sp, ax
    cld

    xor ax, ax
    mov ds, ax      ;DS:SI > 0x0000:0x0001 (0x00001)
    mov bx, ax
    mov ax, 0x0001  ;ES:BX > 0x07e0:0x0000 (0x07e00)
    mov si, ax
    mov ax, 0x07e0
    mov es,ax
    
    Kernel2Memory:
    mov al, 0x01    ; Load 1 sectors
    mov ah, 0x02    ; Load disk data to ES:BX
    mov cl, 0x01    ; Sector = 1
    mov ch, 0x00    ; Cylinder = 0
    mov dh, 0x00    ; Head = 0
    int 13h         ; Read
    jc Kernel2Memory

    jmp 0x07e0:0x0000

    TIMES 510-($-$$) db  0
    dw 0xaa55

    incbin "Kernel.bin"

    TIMES 1024*1440-($-$$) db 0x00

The BIOS Parameter Block which will add to the first of the boot sector (BIOSParameter.inc):

    global bpb_disk_info
    jmp short boot_continue
    nop

    bpb_disk_info:

    ; Dos 4.0 EBPB 1.44MB floppy
    OEMname:           db    "mkfs.fat"  ; mkfs.fat is what OEMname mkdosfs uses
    bytesPerSector:    dw    512
    sectPerCluster:    db    1
    reservedSectors:   dw    1
    numFAT:            db    2
    numRootDirEntries: dw    224
    numSectors:        dw    2880
    mediaType:         db    0xf0
    numFATsectors:     dw    9
    sectorsPerTrack:   dw    18
    numHeads:          dw    2
    numHiddenSectors:  dd    0
    numSectorsHuge:    dd    0
    driveNum:          db    0
    reserved:          db    0
    signature:         db    0x29
    volumeID:          dd    0x2d7e5a1a
    volumeLabel:       db    "NO NAME    "
    fileSysType:       db    "FAT12   "

And the kernel code let's say it print a simple 'A' character (Kernel.bin):

org 0x7e00
mov al,0x41
mov ah,0x0e
mov bl,0x07
mov bh,0x00
int 0x10
times 512-($-$$) db 0

it's prints nothing. I read lots of problems and solutions in StackOverflow and debugged it as much I could (I'm new to this field). I'll be thankful if someone helps me with the problem.

the command which I use:

nasm -fbin Kernel.asm -o Kernel.bin

nasm -DWITH_BPB -f bin Boot.asm -o OS.img

then run it as a floppy disk in VirtualBox

Thanks In Advance