I am following the 10x Cellranger steps and using the same files for cellranger count. I run this from the fastq directory that contains all the PBMCs fastq files and the GRCh38 files too.

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You put a space between --fastqs= and /home/.../pbmc_1k_v3_fastqs, and thus it think you didn't provide an argument after --fastqs. There are two possible syntax:

  • either you want a space, but then don't put a =, as in --fastqs /home/.../pbmc_1k_v3_fastqs;
  • or you put an equal sign, but then don't put a space, as in --fastqs=/home/.../pbmc_1k_v3_fastqs.