Getting this issue while trying to host flask application on IIS

Question

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I was following this https://medium.com/@dpralay07/deploy-a-python-flask-application-in-iis-server-and-run-on-machine-ip-address-ddb81df8edf3

And i get this error when i add the environment variable both pythonpath and wsgi handler and when i press ok in Edit FastCGI Application this error appeared.

So can anyone has solution to this issue i have tried rebooting the server