Is there a way to amplify a sound at a specific frequency using PyAudio?

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I am trying to build an application using pyaudio which can amplify sounds at certain frequencies without affecting sounds at other frequencies.

For example. If I was playing a piece of audio with 2 frequencies. 250Hz and 500Hz. How do I only amplify the sound at 250 Hz.

Any guidance on where to start would be very helpful. Whether its code stubs or where I should begin looking.

Or if there are other python libraries that do this I'd be very grateful to know what they are and how they do this.

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