Goal: mas5 normalize data.
Problem: when I try the following R code, I get this
error: unable to find an inherited method for function bg.correct for signature ExpressionFeatureSet, character
I have looked on SO, and found the following: What does this mean: unable to find an inherited method for function ‘A’ for signature ‘"B"’, but I am not exactly sure how to fix my specific problem and use the mas5 function properly. I have also looked at this affy manual but still stuck...
installpkg("affy")
library('affy')
setwd("/Users/er/Desktop/DesktopFolders/DataSets/CD8Helios/Microarray/CELfiles/CEL")
cel_Files <- list.celfiles()
affyRaw <- read.celfiles(cel_Files)
eset <- mas5(affyRaw)
If you are sure that the
.celfiles were created based on experiments performed on the type of array that works withaffypackage than you should try this workflow usingReadAffyfromaffypackage.However, it might be the case that the
affypackage is not designed for your array type. Then, you should switch to theoligoandoligoClassespackages and normalize with analogous functionrma