PLS model in R is not recognizing one of my variables

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I am working on running a PLS model in R to analyze data I have for FTIR and Enzyme activity. For some reason it is giving me error message that it can't find "Pk_1260" which is one of my columns in my spreadsheet. I have run over analyses on this spreadsheet so I know it is uploaded correctly so I am not sure why I am getting this error.

Let me know if you have ideas how to fix! Thank you!

This is the error code: The exact code is: Error in isTRUE(x) : object 'Pk_1260' not found

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