the timeout period elapsed prior to obtaining a connection from the pool

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We have a web application and we are performing load test of 250 users on the application. Its working perfectly in one environment without any error logs or error generated. and it another environment its not working and throwing below error.

Please note that server specifications are exactly similar for SQL server and Application server. Only there are 2 separate environments. enter image description here We have SQL server Enterprise edition and IIS 8 as application server on both machines. Application and DB server are on separate machines for both the environments. All the settings are similar too.

PS: Please do not suggest anything for closing connections etc. Have already reviewed the code. All the connections are being closed by using keyword and connection.close() method. Its already in place.

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